Effects of prolonged whey protein supplementation and resistance training on biomarkers of vitamin B12 status: A 1-year randomized intervention in healthy older adults (the CALM Study)
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Effects of prolonged whey protein supplementation and resistance training on biomarkers of vitamin B12 status : A 1-year randomized intervention in healthy older adults (the CALM Study). / Greibe, Eva; Reitelseder, Søren; Bechshøft, Rasmus L; Bülow, Jacob; Højfeldt, Grith W.; Schacht, Simon Rønnow; Knudsen, Mads Lind; Tetens, Inge; Ostenfeld, Marie S; Mikkelsen, Ulla R; Heegaard, Christian Würtz; Nexo, Ebba; Holm, Lars.
I: Nutrients, Bind 12, Nr. 7, 2015, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Effects of prolonged whey protein supplementation and resistance training on biomarkers of vitamin B12 status
T2 - A 1-year randomized intervention in healthy older adults (the CALM Study)
AU - Greibe, Eva
AU - Reitelseder, Søren
AU - Bechshøft, Rasmus L
AU - Bülow, Jacob
AU - Højfeldt, Grith W.
AU - Schacht, Simon Rønnow
AU - Knudsen, Mads Lind
AU - Tetens, Inge
AU - Ostenfeld, Marie S
AU - Mikkelsen, Ulla R
AU - Heegaard, Christian Würtz
AU - Nexo, Ebba
AU - Holm, Lars
N1 - CURIS 2020 NEXS 224
PY - 2020
Y1 - 2020
N2 - We investigated the effect of long-term whey supplementation on biomarkers of B12 status in healthy older adults subjected to different schemes of supplements and exercise. The total study population examined at baseline consisted of 167 healthy older adults (age ≥ 65 year) who were randomized to 1-y intervention with two daily supplements of (1) whey protein (3.1 µg B12/day) (WHEY-ALL), (2) collagen (1.3 µg B12/day) (COLL), or (3) maltodextrin (0.3 µg B12/day) (CARB). WHEY-ALL was comprised of three groups, who performed heavy resistance training (HRTW), light resistance training (LITW), or no training (WHEY). Dietary intake was assessed through 3-d dietary records. For the longitudinal part of the study, we included only the participants (n = 110), who met the criteria of ≥ 50% compliance to the nutritional intervention and ≥ 66% and ≥ 75% compliance to the heavy and light training, respectively. Fasting blood samples collected at baseline and 12 months and non-fasting samples collected at 6 and 18 months were examined for methylmalonic acid, B12 and holotranscobalamin. At baseline, the study population (n = 167) had an overall adequate dietary B12 intake of median (range) 5.3 (0.7-65) µg/day and median B12 biomarker values within reference intervals. The whey intervention (WHEY-ALL) caused an increase in B12 (P < 0.0001) and holotranscobalamin (P < 0.0001). In addition, methylmalonic acid decreased in the LITW group (P = 0.04). No change in B12 biomarkers was observed during the intervention with collagen or carbohydrate, and the training schedules induced no changes. In conclusion, longer-term daily whey intake increased plasma B12 and holotranscobalamin in older individuals. No effect of intervention with collagen or carbohydrate or different training regimes was observed. Interestingly, the biomarkers of B12 status appeared to be affected by fasting vs. non-fasting conditions during sample collection.
AB - We investigated the effect of long-term whey supplementation on biomarkers of B12 status in healthy older adults subjected to different schemes of supplements and exercise. The total study population examined at baseline consisted of 167 healthy older adults (age ≥ 65 year) who were randomized to 1-y intervention with two daily supplements of (1) whey protein (3.1 µg B12/day) (WHEY-ALL), (2) collagen (1.3 µg B12/day) (COLL), or (3) maltodextrin (0.3 µg B12/day) (CARB). WHEY-ALL was comprised of three groups, who performed heavy resistance training (HRTW), light resistance training (LITW), or no training (WHEY). Dietary intake was assessed through 3-d dietary records. For the longitudinal part of the study, we included only the participants (n = 110), who met the criteria of ≥ 50% compliance to the nutritional intervention and ≥ 66% and ≥ 75% compliance to the heavy and light training, respectively. Fasting blood samples collected at baseline and 12 months and non-fasting samples collected at 6 and 18 months were examined for methylmalonic acid, B12 and holotranscobalamin. At baseline, the study population (n = 167) had an overall adequate dietary B12 intake of median (range) 5.3 (0.7-65) µg/day and median B12 biomarker values within reference intervals. The whey intervention (WHEY-ALL) caused an increase in B12 (P < 0.0001) and holotranscobalamin (P < 0.0001). In addition, methylmalonic acid decreased in the LITW group (P = 0.04). No change in B12 biomarkers was observed during the intervention with collagen or carbohydrate, and the training schedules induced no changes. In conclusion, longer-term daily whey intake increased plasma B12 and holotranscobalamin in older individuals. No effect of intervention with collagen or carbohydrate or different training regimes was observed. Interestingly, the biomarkers of B12 status appeared to be affected by fasting vs. non-fasting conditions during sample collection.
KW - Faculty of Science
KW - Vitamin B12
KW - Cobalamin
KW - Intervention
KW - Whey
KW - Whey protein hydrolysate
KW - Carbohydrate
KW - Maltodextrin
KW - Collagen
KW - Collagen protein hydrolysate
KW - Exercise
KW - Fasting versus non-fasting blood samples
U2 - 10.3390/nu12072015
DO - 10.3390/nu12072015
M3 - Journal article
C2 - 32645934
VL - 12
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 7
M1 - 2015
ER -
ID: 245234900