Effects of short-term dietary protein restriction on blood amino acid levels in young men
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Effects of short-term dietary protein restriction on blood amino acid levels in young men. / Sjøberg, Kim Anker; Schmoll, Dieter; Piper, Matthew D W; Kiens, Bente; Rose, Adam John.
I: Nutrients, Bind 12, Nr. 8, 2195, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Effects of short-term dietary protein restriction on blood amino acid levels in young men
AU - Sjøberg, Kim Anker
AU - Schmoll, Dieter
AU - Piper, Matthew D W
AU - Kiens, Bente
AU - Rose, Adam John
N1 - CURIS 2020 NEXS 241
PY - 2020
Y1 - 2020
N2 - Pre-clinical studies show that dietary protein restriction (DPR) improves healthspan and retards many age-related diseases such as type 2 diabetes. While mouse studies have shown that restriction of certain essential amino acids is required for this response, less is known about which amino acids are affected by DPR in humans. Here, using a within-subjects diet design, we examined the effects of dietary protein restriction in the fasted state, as well as acutely after meal feeding, on blood plasma amino acid levels. While very few amino acids were affected by DPR in the fasted state, several proteinogenic AAs such as isoleucine, leucine, lysine, phenylalanine, threonine, tyrosine, and valine were lower in the meal-fed state with DPR. In addition, the non-proteinogenic AAs such as 1- and 3-methyl-histidine were also lower with meal feeding during DPR. Lastly, using in silico predictions of the most limiting essential AAs compared with human exome AA usage, we demonstrate that leucine, methionine, and threonine are potentially the most limiting essential AAs with DPR. In summary, acute meal feeding allows more accurate determination of which AAs are affected by dietary interventions, with most essential AAs lowered by DPR.
AB - Pre-clinical studies show that dietary protein restriction (DPR) improves healthspan and retards many age-related diseases such as type 2 diabetes. While mouse studies have shown that restriction of certain essential amino acids is required for this response, less is known about which amino acids are affected by DPR in humans. Here, using a within-subjects diet design, we examined the effects of dietary protein restriction in the fasted state, as well as acutely after meal feeding, on blood plasma amino acid levels. While very few amino acids were affected by DPR in the fasted state, several proteinogenic AAs such as isoleucine, leucine, lysine, phenylalanine, threonine, tyrosine, and valine were lower in the meal-fed state with DPR. In addition, the non-proteinogenic AAs such as 1- and 3-methyl-histidine were also lower with meal feeding during DPR. Lastly, using in silico predictions of the most limiting essential AAs compared with human exome AA usage, we demonstrate that leucine, methionine, and threonine are potentially the most limiting essential AAs with DPR. In summary, acute meal feeding allows more accurate determination of which AAs are affected by dietary interventions, with most essential AAs lowered by DPR.
KW - Faculty of Science
KW - Amino acids
KW - Dietary protein
KW - Meal feeding
KW - Fasting
KW - Restriction
U2 - 10.3390/nu12082195
DO - 10.3390/nu12082195
M3 - Journal article
C2 - 32717986
VL - 12
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 8
M1 - 2195
ER -
ID: 245414819