Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Measuring the kinase activity of the 5'-AMP-activated protein kinase (AMPK) is an essential part of understanding the regulation of this metabolic master switch. The AMPK heterotrimer can exist in 12 different constellations with potentially diverse activation patterns. It is therefore important to be able to measure heterotrimer-specific activity to discriminate between these patterns. In this chapter we describe how to measure the AMPK activity of specific heterotrimeric complexes by consecutive immunoprecipitations and how the assay can be performed in a medium throughput fashion using 96-well plates.
Originalsprog | Engelsk |
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Titel | AMPK : Methods and Protecols |
Redaktører | Dietbert Neumann, Benoit Viollet |
Antal sider | 14 |
Udgivelsessted | New York |
Forlag | Humana Press |
Publikationsdato | 2018 |
Sider | 215-228 |
Kapitel | 14 |
ISBN (Trykt) | 978-1-4939-7597-6 |
ISBN (Elektronisk) | 978-1-4939-7598-3 |
DOI | |
Status | Udgivet - 2018 |
Navn | Methods in Molecular Biology |
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Nummer | 1732 |
ISSN | 1064-3745 |
Bibliografisk note
CURIS 2018 NEXS 080
- Det Natur- og Biovidenskabelige Fakultet
Forskningsområder
ID: 191294168