The effect of casein, hydrolyzed casein, and whey proteins on urinary and postprandial plasma metabolites in overweight and moderately obese human subjects
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The effect of casein, hydrolyzed casein, and whey proteins on urinary and postprandial plasma metabolites in overweight and moderately obese human subjects. / Schmedes, Mette S; Bendtsen, Line Quist; Gomes, Sisse; Liaset, Bjørn; Holst, Jens Juul; Ritz, Christian; Reitelseder, Søren; Sjödin, Anders Mikael; Astrup, Arne; Young, Jette F; Sundekilde, Ulrik K; Bertram, Hanne C.
I: Journal of the Science of Food and Agriculture, Bind 98, Nr. 15, 2018, s. 5598-5605.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - The effect of casein, hydrolyzed casein, and whey proteins on urinary and postprandial plasma metabolites in overweight and moderately obese human subjects
AU - Schmedes, Mette S
AU - Bendtsen, Line Quist
AU - Gomes, Sisse
AU - Liaset, Bjørn
AU - Holst, Jens Juul
AU - Ritz, Christian
AU - Reitelseder, Søren
AU - Sjödin, Anders Mikael
AU - Astrup, Arne
AU - Young, Jette F
AU - Sundekilde, Ulrik K
AU - Bertram, Hanne C
N1 - CURIS 2018 NEXS 210
PY - 2018
Y1 - 2018
N2 - Background: Casein and whey proteins differ in amino acid composition and absorption rate; however the absorption rate of casein can be increased to mimic that of whey proteins by exogenous hydrolysis. In view of these compositional differences we studied the metabolic responses to intake of casein, hydrolyzed casein and whey proteins in overweight and moderately obese men and women by investigating select urinary and blood plasma metabolites.Results: A total of 21 urinary and 23 plasma metabolites were identified by NMR spectroscopy. The postprandial plasma metabolites revealed a significant diet-time interaction for isoleucine (P = 0.001) and tyrosine (P = 0.001). The level of isoleucine and tyrosine peaked 90 min postprandial with a 1.4-fold difference following intake of whey proteins compared to casein and hydrolyzed casein, respectively. A 1.2-fold higher urinary level of lactate was observed after intake of whey proteins compared to intake of intact casein (P < 0.01).Conclusions: The plasma metabolites revealed different amino acid profiles reflecting the amino acid composition of casein and whey proteins. Furthermore, the results support that casein hydrolysates do neither affect the postprandial amino acid absorption rate nor the amino acid level compared to that of intact casein. The urinary lactate increases following whey protein intake might indicate a higher metabolism of glucogenic amino acids.
AB - Background: Casein and whey proteins differ in amino acid composition and absorption rate; however the absorption rate of casein can be increased to mimic that of whey proteins by exogenous hydrolysis. In view of these compositional differences we studied the metabolic responses to intake of casein, hydrolyzed casein and whey proteins in overweight and moderately obese men and women by investigating select urinary and blood plasma metabolites.Results: A total of 21 urinary and 23 plasma metabolites were identified by NMR spectroscopy. The postprandial plasma metabolites revealed a significant diet-time interaction for isoleucine (P = 0.001) and tyrosine (P = 0.001). The level of isoleucine and tyrosine peaked 90 min postprandial with a 1.4-fold difference following intake of whey proteins compared to casein and hydrolyzed casein, respectively. A 1.2-fold higher urinary level of lactate was observed after intake of whey proteins compared to intake of intact casein (P < 0.01).Conclusions: The plasma metabolites revealed different amino acid profiles reflecting the amino acid composition of casein and whey proteins. Furthermore, the results support that casein hydrolysates do neither affect the postprandial amino acid absorption rate nor the amino acid level compared to that of intact casein. The urinary lactate increases following whey protein intake might indicate a higher metabolism of glucogenic amino acids.
KW - Faculty of Science
KW - Dairy proteins
KW - Absorption kinetics
KW - Metabolomics
KW - Postprandial metabolism
U2 - 10.1002/jsfa.9103
DO - 10.1002/jsfa.9103
M3 - Journal article
C2 - 29696654
VL - 98
SP - 5598
EP - 5605
JO - Journal of the Science of Food and Agriculture
JF - Journal of the Science of Food and Agriculture
SN - 0022-5142
IS - 15
ER -
ID: 195968637